Background. The infl uence of weight loss treatment on sex hormones profi le has been studied mainly in
women with polycystic ovary syndrome (PCOS), but in obese premenopausal women without PCOS it still
remains unclear. The aim of the study was to evaluate the eff ect of two approaches to obesity treatment on the
serum level of sex hormones in obese women of child-bearing age without PCOS.
Material and methods. 77 obese Caucasian women (aged 31.2 ±8.3 years) were randomized into two
groups: 39 women received a low-calorie diet (LC) and 38 received an isocaloric diet plus metformin (IM),
for 12 weeks. Anthropometric parameters, body composition and serum concentrations of estradiol (E2),
testosterone (T), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and dehydroepiandrosterone
(DHEA-S) sulfate were evaluated at baseline and after the study.
Results. Reductions in body weight, body mass index (BMI), waist and body fat content with an increase
in lean body percent were signifi cant and comparable between the LC and IM group after the trial. The concentrations
of serum FSH, LH, E2, DHEA and T did not change in either group after treatment. A tendency
towards an increase in the E2 concentration in both groups and a decrease in the T level in the LC group
was observed. The correlations between a change in BMI, fat content, waist-hip ratio and a change in T were
documented in the LC group.
Conclusion. A 12-week low-calorie diet and an isocaloric diet combined with metformin produced comparable
and signifi cant weight loss with improvements in body composition. Both interventions did not signifi cantly
aff ect FSH, LH and DHEA sulfate serum concentrations, only a trend towards an E2 increase and a T decrease
was observed, stronger in LC group. The signifi cant correlations shown between the changes in anthropometric
and body composition parameters and T serum levels in women treated with a low-calorie diet alone show the
benefi cial eff ect of a lifestyle intervention on the sex hormone in obese premenopausal women.